Figure 6. Carvacrol inhibits the native TRPL channels in photoreceptor cells.
A. Representative whole-cell recordings of the light induced current (LIC) from isolated Drosophila ommatidia of the trpP343 mutant.
left, Control responses (middle trace) to a train of orange light pulses (top trace) of constant intensity (−logI=3.0, n=5). The bottom two traces show the waveform of the LICs in a faster time scale. right, The paradigm of the left panel was repeated, except that carvacrol (500μM) was applied as indicated (n=5).
B. The averaged peak amplitude of the light responses (such as in (A)) is plotted as a function of time (error bars are SEM, n=5).
C. The paradigm of (A) was repeated, except that wt flies were used and a larger dark interval was interposed after the second light pulse (n=5).
D. Histogram presenting the averaged and normalized peak amplitude of light responses after 6 min in the dark following application of carvacrol containing solutions (carvacrol, red) as compared to experiments in which carvacrol was not applied (control, black, error bars are SEM, n=5)