Fig. 2.
MPL1886InsGG is hypersensitive to thrombopoietin and transforms BA/F3 cells. (A) BA/F3 cells were plated in a dose gradient of thrombopoietin. After 3 days, cell proliferation was assessed by using an MTS assay. Values represent percentage mean (normalized to 10 ng/mL TPO wells) ± SEM (n = 6). *, P < 0.05. (B) BA/F3 cells were stimulated for 15 min with 0–10 ng/mL thrombopoietin. Whole-cell lysates were subjected to immunoblotting with antibodies specific for total (Middle) or phospho-STAT5 (Top), as well as β-actin (Bottom). (C) BA/F3 cells were plated in medium lacking WEHI-conditioned medium, and total viable cells were counted daily for 1 week. Values represent mean viable cells ± SEM (n = 3). *, P < 0.05.