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. Author manuscript; available in PMC: 2009 May 11.
Published in final edited form as: Nat Cell Biol. 2008 May 18;10(6):643–653. doi: 10.1038/ncb1727

Figure 7.

Figure 7

Silencing of Huwe1 blocks differentiation of neural progenitors in vivo. (a) Ex vivo electroporation of control, N-myc, Huwe1 and Huwe1 + N-myc siRNA into E13.5 mouse cortices followed by organotypic slice culture for 1.5 days. Cortical slices were double-labelled with Neurogenin2 (Ngn2, red) and GFP (green) to identify transfected cells. (b) Quantification of GFP-positive/Ngn2-positive cells. (c) Cortical slices were double-labelled with the early neuronal marker HuC/D (red) and GFP (green). (d) Quantification of GFP-positive/HuC/D-positive cells. (e) Cortical slices were double-labelled with the mature neuronal marker Tuj1 (red) and GFP (green). (f) Quantification of GFP-positive/Tuj1-positive cells. Results shown in b, d and f are mean ± s.e.m. of four sections for Ngn2, three sections for HuC/D and three sections for TuJ1 from one experiment; *P < 0.05, **P < 0.01, Student's t-test). Arrowheads indicate GFP-positive/Cy3-positive cells; arrows indicate GFP-positive/Cy3-negative cells. Scale bars are 50 μm. IZ, intermediate zone; VZ, ventricular zone.