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. Author manuscript; available in PMC: 2009 May 11.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Nov 1;875(1):304–316. doi: 10.1016/j.jchromb.2008.06.028

Fig. 7.

Fig. 7

Response surface plots of multivariate optimization of mobile phase parameters for the resolution of the first two enantiomers of nadolol and the overall run time. Optimum conditions shown by arrows of 40/60/1.6/0.4 MeOH/ACN/HOAc/TEA were determined from the Design Expert software. RSMs for resolution N1–N2 and overall runtime holding: (A) TEA constant at 0.4%, (B) HOAc constant at 1.6%, and (C) MeOH/ACN constant at 40/60% while varying the other two variables.