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. Author manuscript; available in PMC: 2009 May 11.
Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Nov 1;875(1):304–316. doi: 10.1016/j.jchromb.2008.06.028

Table 1.

Most important D-optimal experimental design factors showing high and low value restraints for the design space used for the optimization of mobile phase composition, sheath liquid composition, and spray chamber parameters

Mobile phase optimization Levels
Design factors
 +1
 −1
F1: methanol/acetonitrile (v/v) 40/60 30/70
F2: acetic acid (%) 0.1 1.6
F3: triethylamine (%) 0.1 0.4
Sheath liquid optimization Levels
Design factors
 +1
 −1
F4: methanol/H2O (v/v) 98/2 20/80
F5: acetic acid (%) 2.00 0.5
Spray chamber optimization Levels
Design factors
 +1
 −1
F6: drying gas flow rate (L/min) 6 3
F7: drying gas temperature (°C) 220 120
F8: nebulizer pressure (psi) 5.0 2.0