Table 2.

The resolution and the total runtime data gathered for the D-optimal experimental design run order for multivariate optimization of mobile phase parameters

Run	Expermental parameters			Experimental responses
	MeOH/CAN (%v/v)	%HOAc	%TEA	Resolution							Run time (min)
				N1-N2	N2-N3	N3-N4	N4-L1	L1-L2	L2-L3	L3-L4
1	32.5	1.225	0.25	1.0	2.0	2.9	6.7	1.8	1.9	1.3	81
2	35	1.6	0.1	0.9	2.1	3.1	8.0	2.0	2.3	1.7	79
3	30	0.85	0.1	0.8	1.4	2.9	5.8	1.6	1.4	1.1	92
4	40	1.6	0.1	0.8	2.1	2.6	8.6	2.0	3.1	2.5	78
5	30	0.1	0.4	0.6	1.1	2.1	4.5	0.7	1.1	0.0	66
6	30	1.6	0.4	0.8	2.0	2.9	5.2	1.9	2.2	1.3	75
7	40	1.6	0.4	0.9	2.2	2.7	12	2.0	2.4	1.7	56
8	30	0.1	0.4	0.7	1.1	2.3	6.0	0.3	0.6	0.0	62
9	40	0.1	0.4	0.4	0.9	0.9	0.8	1.9	2.7	0.0	51
10	40	1.6	0.4	1.0	2.3	3.0	12	2.1	2.7	1.7	55
11	40	0.1	0.1	1.2	2.4	3.2	9.0	2.2	2.8	1.6	123
12	40	0.85	0.25	1.0	2.2	2.9	11	1.9	1.9	1.5	72
13	35	0.85	0.4	0.8	1.7	2.4	5.5	1.8	1.5	0.8	70
14	40	0.1	0.4	0.4	0.9	0.6	0.8	1.8	2.6	0.0	51
15	30	1.6	0.4	0.9	1.8	2.8	5.9	1.8	1.8	1.1	73
16	35	0.1	0.1	1.3	2.3	3.6	11	2.1	2.3	2.2	142
17	30	0.1	0.1	1.0	1.8	2.9	6.5	2.2	1.3	1.2	159
18	30	1.6	0.1	0.8	1.6	2.3	6.0	1.7	1.4	1.4	90
19	35	0.1	0.25	0.9	2.1	2.9	0.0	1.6	1.1	0.0	92
20	35	0.85	0.4	0.8	1.6	2.3	5.2	1.7	1.7	1.2	68