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Effects of naloxone on the behavioral seizure score increased by i.c.v. morphine 100 μg or i.c.v. DAMGO 1.5 μg. Naloxone 1 mg/kg was administered subcutaneously 15 min before administration of morphine or DAMGO. Each animal was scored according to the highest class of behavioral manifestation observed during the 1 h observation period. Each value represents mean ± S.E.M. (n=6).**, P<0.01 compared with the Veh+Veh condition; #, P<0.05 compared with Veh+(morphine or DAMGO).
