Fig. 4.

Empirically determined plane of section in three experimental brains compared to the plane of section in a reference Atlas (Swanson, 2004). Although these experimental brains were prepared carefully in a brain blocker (Swanson, 2004), the actual plane of section was clearly different in each one, as determined by the method illustrated in Figure 2, and described in the text. The case where the atlas plane of section equals a (hypothetical) experimental brain plane of section is shown in A and B, where the atlas midsagittal projection was scaled to match the dimensions of the experimental brain (as in Fig. 2B). For each brain the plane of section is shown as viewed from the side (sagittal view: A, C, E, G) and from the top (dorsal view: B, D, F, H).