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The Q/N-rich region from Ccr4p aggregates in cytoplasmic foci and responds to stress. (A) Localization of GFP-tagged Q/N-rich regions of Ccr4p (aa 1-229; pMR202), Pop2p (aa 1-156; pMR203) and Dhh1p (aa 427-506; pMR204) before and after hypo-osmotic shock (B) GFP-Ccr4(1-229) aggregates localize to the cytoplasm as shown in these fixed cells with DAPI stained nuclear DNA (C) The majority of GFP-Ccr4(1-229) aggregates do not co-localize with Dcp2-RFP (pRP1155) foci after osmotic shock.
