TABLE 1.
Permeabilities of [14C]mannitol, [3H]prazosin, [14C]zidovudine, [3H]abacavir, and [14C]imatinib in MDCK wild-type and MDCK Bcrp1 cells
Papp, calculated apparent permeabilities from eq. 1; efflux ratio (ER) = Papp B-to-A transport/Papp A-to-B transport.
|
Substrate
|
MDCK WT
|
MDCK Bcrp1
|
||||
|---|---|---|---|---|---|---|
| A-to-B Papp | B-to-A Papp | ER | A-to-B Papp | B-to-A Papp | ER | |
| ×107 cm/s | ×107 cm/s | |||||
| Mannitol | 11 ± 3 | 11 ± 1 (N.S.) | 1 | 8 ± 3 | 7 ± 2 (N.S.) | 0.9 |
| Prazosin | 33 ± 11 | 43 ± 7 (N.S.) | 1.3 | 5 ± 4 | 57 ± 27* | 10.5 |
| Zidovudine | 14 ± 1 | 12 ± 1 (N.S.) | 0.9 | 5 ± 1 | 44 ± 1* | 10 |
| Abacavir | 100 ± 43 | 129 ± 31 (N.S.) | 1.3 | 7 ± 4 | 187 ± 12* | 27 |
| Imatinib (STI-571) | 29 ± 8 | 52 ± 3* | 1.8 | 3 ± 1 | 181 ± 9* | 62 |
N.S., not significantly different from apical-to-basolateral transport.
*
Significantly different from apical-to-basolateral transport.