TABLE 2.
Bile acid composition of bile acid pool
Fxr-null and wild-type mice were fed a control diet containing 0.5% CA or 0.5% CA and 1.25% Chol (CA+Chol) for 6 days. Liver, gallbladder, and small intestine were isolated, and bile acids were extracted. Bile acid pool size was analyzed by HPLC. The data are shown as the mean ± S.D. from five mice. TLCA, LCA, βMCA, UDCA, TCDCA, and CDCA were below detection limits (0.1 μmol/100 g b.wt.).
| TCA + CA | TDCA + DCA | TβMCA + βMCA | TUDCA + UDCA | Total | |
|---|---|---|---|---|---|
| μmol/100 g b.wt. | μmol/100 g b.wt. | μmol/100 g b.wt. | μmol/100 g b.wt. | μmol/100 g b.wt. | |
| Wild-type (CA) | 95.9 ± 11.0 | 27.9 ± 16.0 | 1.1 ± 1.5 | 4.0 ± 2.9 | 128.9 ± 15.1 |
| Wild-type (CA+Chol) | 79.7 ± 12.8 | 29.7 ± 4.9 | 0.3 ± 0.5 | 4.1 ± 1.4 | 113.9 ± 13.3 |
| Fxr-null (CA) | 96.4 ± 26.1 | 6.1 ± 3.4 | 1.3 ± 1.0 | 5.8 ± 1.8 | 109.6 ± 26.2 |
| Fxr-null (CA+Chol) | 55.5 ± 12.6a | 5.8 ± 2.8 | 0.2 ± 0.2 | 3.9 ± 1.8 | 65.4 ± 16.6a |
a
p < 0.05 versus corresponding CA group.