TABLE 2.
Reversal of cytotoxicity in ABCG2-expressing cells by sunitinib
|
Cell Line
|
Topotecan,
IC50a
(Relative
Resistance)b
|
SN-38,
IC50a
(Relative
Resistance)b
|
||
|---|---|---|---|---|
| Control (DMSO) | +2 μM Sunitinib | Control (DMSO) | +2 μM Sunitinib | |
| μM | ||||
| pcDNA3.1-HEK | 0.011 ± 0.0041 | 0.014 ± 0.0078 | 0.0017 ± 0.0011 | 0.0026 ± 0.0017 |
| Arg482-HEK (ABCG2) | 0.21 ± 0.0066 | 0.019 ± 0.0036 | 0.048 ± 0.0038 | 0.0022 ± 0.00047 |
| (19) | (1.7) | (28) | (1.3) | |
DMSO, dimethyl sulfoxide.
a
The values represent the mean ± S.D. of three independent experiments performed in triplicate.
b
Relative resistance values (in parentheses) were obtained by dividing the IC50 value of the Arg482-HEK293 cells by the IC50 value of the empty vector pcDNA3.1-transfected HEK cell line.