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HPLC traces obtained from CYP2C9-mediated conversion of warfarin to the 7-hydroxy metabolite (inset). Curve a wash obtained from solution-based incubation containing NADPH and CPR, and curve b by a controlled potential electrolysis (–0.38 V) at a Au-CYP2C9 electrode in the absence of NADPH and CPR. The dashed traces, offset above curves a and b, are the controls and were obtained on incubation mixtures that lacked NADPH (above curve a) or no current was applied (above curve b).
