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Enzyme kinetics of dopamine (DA) glucuronidation by four mutants of human UGT1A10. Samples were incubated in the presence of 1 mM UDPGA and 100 to 400 μg/ml protein at 37°C for 45 min. All samples were prepared in triplicate. The normalized initial rate data were fitted to the Michaelis-Menten (10F93A and 10F93L) or Hill equation (10F90A and 10F90L). The enzyme kinetic constants are listed in Table 1.
