BSA is required for optimal bilirubin production at subsaturating and
saturating CPR levels. A, BSA titration of a bilirubin assay at 1:5 CPR/HO-1
(□, dashed line) and 2:1 CPR/HO-1 (▴, solid line). B, real-time
kinetic traces of bilirubin production with 0 mg/ml BSA, 1:5 CPR/HO-1
(——); 0 mg/ml BSA, 2:1 CPR/HO-1 (— ··
—); 0.25 mg/ml BSA, 1:5 CPR/HO-1 (— · —); and 0.25
mg/ml BSA, 2:1 CPR/HO-1 (- - -). HO-1 concentration was 0.05 μM for all the
assays. Values in A represent the mean ± S.E. of n = 3.