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BVR titration and real-time curves of bilirubin production. A, BVRs ranging from 0 to 0.1 μM were analyzed to determine the optimal concentration for bilirubin production at subsaturating and saturating CPR concentrations: 1:5 CPR/HO-1 (□) and 2:1 CPR/HO-1 (▴). B, real-time bilirubin assay curves of the four highest BVR concentrations (0.01–0.1 μM). HO-1 concentration was 0.05 μM for all the assays. Values in A represent the mean ± S.E. of n = 3.
