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Effect of SOD and catalase on the linearity of the bilirubin formation real-time activity curves. A and B, increasing amounts of superoxide dismutase (0, 0.00025, 0.00125, 0.00625, 0.025, 0.25, 0.625, and 1.25 U/μl) were added to the reaction mixture containing 1:5 (A) and 2:1 (B) CPR/HO-1. C and D, catalase (0, 0.00025, 0.00625, 0.025, 0.25, 0.625, 1.25, and 2.5 U/μl) was titrated into the 1:5 (C) and 2:1 (D) CPR/HO-1 reactions. HO-1 concentration was 0.05 μM for all the reactions.
