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. Author manuscript; available in PMC: 2010 Jan 20.
Published in final edited form as: Biochemistry. 2009 Jan 20;48(2):289–301. doi: 10.1021/bi8015668

Figure 2.

Figure 2

The left column panels are HPLC chromatograms obtained during peptide purification and correspond to the following syntheses: a, HFPmn; c, HFPdm; e, HFPdm(Cys); g, HFPtr; and i, HFPte. The top, middle, and bottom chromatograms in panel g are for syntheses with HFPtr cross-linking times of 0.5, 1.5, and 2.5 hours, respectively. The vertical scales in panels a, c, e, and i are A214 and the vertical scale in panel g is A280. In panels c and i, the large peaks at 8 minutes are due to DMAP. For a given chromatogram in the left column panel, the HPLC fraction marked with an asterisk was analyzed by MALDI-TOF mass spectroscopy and the corresponding mass spectrum is displayed in the right column panel. The spectral peaks marked with asterisks are discussed in the main text.