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. Author manuscript; available in PMC: 2010 Jan 20.
Published in final edited form as: Biochemistry. 2009 Jan 20;48(2):289–301. doi: 10.1021/bi8015668

Figure 4.

Figure 4

Panel a displays 13C-detect/31P-dephase REDOR S0 and S1 spectra for PC:PG-associated HFPs and panel b displays spectra for PC:PG:CHOL-associated HFPs. The dotted lines are added to provide easier visual comparison of the S0 and S1 intensities. The samples for the top, middle, and bottom spectra contained HFPmn-A6, HFPdm-A6, and HFPtr-A6, respectively, and the HFP:lipid mol ratios were 0.040, 0.020, and 0.013. Each spectrum was obtained with 24 ms dephasing time and was processed with 300 Hz Gaussian line broadening and polynomial baseline correction. For panel a, the numbers of scans summed for each S0 and S1 spectrum were: HFPmn-A6, 40832; HFPdm-A6, 74756; and HFPtr-A6, 20736. For panel b, the numbers of scans were: HFPmn-A6, 28288; HFPdm-A6, 85136; and HFPtr-A6, 29696. Panels c and d display plots of (ΔS/S0)exp vs dephasing time with color coding: HFPmn (black); HFPdm (red); and HFPtr (blue). A 1σ error bar is displayed for each point and lines between points are displayed for visual clarity. Each (ΔS/S0)exp value was determined from integrations of 1 ppm regions of the S0 and S1 spectra. In the panel c plot, the integration was centered at the ~180 ppm peak for samples containing PC:PG and for the panel d plot, the integration was centered at the ~175 ppm peak for samples containing PC:PG:CHOL. These two peaks are respectively assigned to helical and β strand A6 conformations, respectively.