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. Author manuscript; available in PMC: 2010 Jan 20.
Published in final edited form as: Biochemistry. 2009 Jan 20;48(2):289–301. doi: 10.1021/bi8015668

Table 2.

Fitting parameters for the lipid mixing kinetics at 35°C a,b

Construct k1
(10−3 s−1)		 k2
(10−3 s−1)		 F0
(a.u.)		 F1
(a.u.)		 F2
(a.u.)		 Eac
(kJ/mol)		 ln A ΔS‡d
(J/mol-K)
HFPdm 54.9(0.3) 4.9(0.2) 0.5(0.1) 19.6(0.3) 19.8(0.1) 40.6(3.4) 13.2(1.2) −152
HFPtr 139(3) 17.3(0.3) 0.9(0.2) 50.1(0.2) 50.0(0.2) 25.8(1.2) 8.0(0.8) −195
HFPte 185(7) 20.7(0.3) 0.9(0.3) 49.8(0.3) 50.1(0.2) 20.1(0.7) 6.2(0.5) −210
a

Fitting uncertainties are given in parentheses. The variation of a parameter value from fitting data of different runs was less than the fitting uncertainty of a single run.

b

The k1, k2, F0, F1 and F2 were obtained using Eq. 2 in the main text.

c

Ea and ln A were calculated using ln k1 = ln A – Ea/RT and k1 values from temperatures between 25 and 40 °C.

d

ΔS‡ was calculated using ΔS‡ = R × [ln (Ah/kBT) – 2].