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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Cancer Prev Res (Phila). 2008 Nov;1(6):485–493. doi: 10.1158/1940-6207.CAPR-08-0149

Figure 4. HSP90 inhibitors suppress B[a]P- and TCDD-mediated induction of CYP1A1 and CYP1B1 protein.

Figure 4

MSK-Leuk1 cells were treated with the indicated concentrations of 17-AAG or celastrol for 2 h. Subsequently, cells received vehicle, 1 μmol/L B[a]P or 5 nmol/L TCDD for 5 h, and were then harvested for Western blot analysis. Cellular lysate protein (100 μg/lane) was loaded onto a 10% SDS–polyacrylamide gel, electrophoresed and subsequently transferred onto nitrocellulose. Immunoblots were probed with antibodies specific for CYP1A1 (panel A), CYP1B1 (panel B) and β-actin.