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. 2009 Apr 17;10(4):1683–1696. doi: 10.3390/ijms10041683

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© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 3. — Changes in f upon addition of anti-biotin (t₁ = 37 min) to an SLB from vesicles with biotin-lipids selectively incorporated in the outer leaflet. The exposure was followed by rinsing (t₂ = 77 min) and addition of free biotin (t₃ = 86 min) to promote removal of bound anti-biotin. Also shown is a second addition of anti-biotin (t₄ = 1039 min) followed by rinsing (t = 1079 min) and addition of free biotin (t = 1085 min).