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. 2009 Apr 21;10(4):1788–1807. doi: 10.3390/ijms10041788

Figure 4.

Figure 4.

RT-PCR analysis of the products of template-directed recombination of M2- RNA and HIV-RNA. Autoradiogram of a 10% denaturing PAAG. “Incubated” group of lanes: RT-PCR analysis of reaction mixtures incubated under conditions of cleavage/ligation reaction at 37 °C for 3 days. 1) positive control (PCR with primers Mfor and Mrev); 2), 3), 4): PCR with primers Hfor and Mrev, using dilutions of cDNA mixture 1:1, 1:10−3 and 1:10−6, respectively. “NI” group: reaction mixture without incubation was used for RT-PCR analysis. 1) positive control (PCR with primers Mfor and Mrev); 2) PCR with primers Hfor and Mrev, and non-diluted cDNA mixture. “Control” group: RT-PCR was performed in the absence of RNA (1) or in the absence of cDNA (2 and 3) with PCR primers Hfor and Mrev (1 and 2) and Mfor and Mrev (3). “L”: DNA ladder (product of partial hydrolysis of M2–80 PCR product at adenine and guanine sites). Location of major products in gel is marked with arrows. Mrev primer was 5′-32P-labelled in all reaction mixtures.