Figure 3.

Assays of proteinase inhibitory activities of transgenic SaPIN2a.

(a) Inhibition of trypsin by total soluble proteins (TSP) from leaves of tobacco plants. (b, c) Inhibition trypsin-like proteinases from H. armigera (b) and S. litura (c) midguts by TSP from leaves of tobacco plants. Buffer, extraction buffer for tobacco TSP; WT, leaves of wild-type tobacco plants (negative control); B41, transgenic plant line transformed with pBI121 vector only (negative control); P51, transgenic plant line transformed with pARTSaf; S14, transgenic plant lines transformed with pSa7; F161, transgenic plant lines transformed with pF121. Mean values plus standard errors are given. ** indicates significance at the 1% level.