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. Author manuscript; available in PMC: 2009 May 12.
Published in final edited form as: Immunity. 2007 Jul 19;27(1):135–144. doi: 10.1016/j.immuni.2007.05.022

Figure 1. LPS induces formation of autophagosomes.

Figure 1

(A) RAW264.7 cells were incubated in the absence or presence of LPS for 16 h, fixed, stained with DAPI to visualize the nuclei (blue), and immunolabled with anti- LC3 antibody followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (green). Representative images are shown. (B) Cell viability analysis of RAW264.7 cells, stably expressing LC3-GFP, following incubation in the presence or absence of LPS (100 ng/ml) for 16 h. Cell viability was determined using Cell Viability Analyzer (Vi-Cell, BECKMAN COULTER) based on trypan blue reagent (Mean±SEM, n=4).