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. Author manuscript; available in PMC: 2009 May 12.
Published in final edited form as: Immunity. 2007 Jul 19;27(1):135–144. doi: 10.1016/j.immuni.2007.05.022

Figure 7. p38 MAPK inhibition blocks LPS-induced co-localization of Mycobacterium tuberculosis with autophagosomes.

Figure 7

RAW264.7 cells stably expressing GFP-LC3 were infected with Mycobacterium tuberculosis expressing red fluorescent protein (RFP) 1 h prior to a 30 min incubation with p38 MAPK inhibitor or vehicle (DMSO) followed by further incubation for 16 h in the presence or absence of LPS. Upper panel: representative fluorescence images. Lower panel: Quantitation of percentage of colocalization of Mycobacterium tuberculosis with GFP-LC3-positive autophagosomes GFP-LC3-positive autophagosomes. Graph represents the quantitation of 100 internalized mycobacteria per experimental condition. Data denote means ± SEM from two independent experiments. Scale bar, 10 μm. *denotes p< 0.05, when compared to control condition.