Figure 3. Conservation of the Regulation of DGCR8.

(A) RT-PCR and western blot analysis after mouse Drosha and mouse DGCR8 depletion. Total RNA and cell extract of NIH 3T3 cells were prepared 72 hr siRNA transfection.

(B) RT-PCR analysis after Drosha knockdown in D. melanogaster S2 cells. Total RNA of S2 cells was prepared 7 days after addition of long doublestranded RNAs that target fly Drosha (dsDrosha). Reverse transcription was carried out with random primer. Pri-Bantam was amplified as a positive control.