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. Author manuscript; available in PMC: 2010 Jan 9.
Published in final edited form as: Cell. 2009 Jan 9;136(1):75–84. doi: 10.1016/j.cell.2008.10.053

Figure 6. Expression of Drosha and DGCR8 in Dgcr8 KO Mouse ES Cells.

Figure 6

(A) Western blot analysis of Drosha in mouse ES cells. Δ/flox indicates heterozygote cells, and Δ/Δ indicates homozygous null cells. Two different KO lines (c1 and c2) were used for the experiments (lanes 3 and 4). The Dicer KO ES line (dicer Δ/Δ) was used as a control (Babiarz et al., 2008).

(B) Quantitation of the Drosha mRNA by Affymetrix chip analysis. Three independent experiments were performed (mean ±SD). The methods are described in Wang et al. (2008b).

(C) Western blot analysis of DGCR8 in mouse ES cells. The asterisk represents a nonspecific band which serves as a loading control.

(D) Quantitative real time PCR (qRT-PCR) of the DGCR8 mRNA in Dgcr8 KO mES cell. The relative DGCR8 mRNA levels were determined in wild-type (flox/flox) and heterozygote (Δ/flox). Three independent experiments were performed for quantification (mean ±SD).