Fig. 4. Npc1 mutation- or cholesterol-mediated Ctsk induction is inhibited by chemical p38 inhibition or genetic p38 deletion.
mRNA levels measured in ConA-elicited peritoneal macrophages by quantitative PCR and normalized to ribosomal 36B4. A, C. Cells treated with different inhibitors (10 uM) or DMSO for 24 hrs. *P<0.05 vs. wt/DMSO, #P<0.05 vs. Npc1−/−/DMSO. B. Cells treated with 10 uM SB202190 for indicated time. D, E. wt or p38α−/− cells treated with 50 ug/ml AcLDL±10 ug/ml ACAT inhibitor 58035 for 18 hrs, 5 mM CD-chol (2.5:1, M:M) for 24 hrs, or untreated (CTR). *P<0.05 vs. wt CTR, #P<0.05 vs. wt-CD-chol, §P<0.05 vs. p38α−/− CTR.