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. Author manuscript; available in PMC: 2009 Oct 15.
Published in final edited form as: Cancer Res. 2009 Apr 7;69(8):3374–3381. doi: 10.1158/0008-5472.CAN-08-4290

Figure 1.

Figure 1

Effect of thrombin and serum on tumor cell cycle kinetics in LNCaP prostate and TRAMP tumor cell lines. Androgen-dependent LNCaP prostate (A and B, representative of eight experiments) and TRAMP (C and D, representative of four experiments) were assayed for BrdUrd incorporation and propidium iodide staining. Cells were serum starved for 72 h before repletion with serum or 0.5 unit/mL thrombin. PI-Area, propidium iodide staining of DNA during various phases of the cell cycle. BrdUrd-FITC is a measure of the kinetics of DNA synthesis of the various phases. Note increase in S and decrease in G0-G1 after serum or thrombin addition.