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. Author manuscript; available in PMC: 2009 May 12.
Published in final edited form as: Cell Host Microbe. 2008 Mar 13;3(3):158–167. doi: 10.1016/j.chom.2008.02.002

Figure 2.

Figure 2

Tat-mediated superinduction of NF-κB-responsive genes requires SIRT1

(A) Western blot analysis of SIRT1, Tat and β-actin in SIRT1−/− MEF cells infected with MSCV-based retroviral particles expressing SIRT1 or Tat.

(B–D) Real-time RT-PCR analysis of IκBα (B), E-selectin (C), and GAPDH (D) mRNA levels in the four polyclonal MEF populations described in (A) after incubation with TNFα (20 ng/ml) for 2 h and 8 h. Data are presented relative to values obtained in cells lacking Tat (100%). The mean of three independent experiments (±SEM) is shown.