Tat neutralizes the inhibitory effect of SIRT1 on NF-κB-responsive promoters
Promoter reporter assays using (A) the IκBα, (B) the E-selectin, and (C) the 3X-κB luciferase reporter construct. Luciferase constructs (0.2 μg) were transiently cotransfected with expression vectors for p65 or p65 K310R (1 ng), SIRT1 or SIRT1 P447E (0.2 μg), and Tat (50 ng) into HeLa cells. The mean of three independent experiments (±SEM) is shown.