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. Author manuscript; available in PMC: 2009 May 12.
Published in final edited form as: Cell Host Microbe. 2008 Mar 13;3(3):158–167. doi: 10.1016/j.chom.2008.02.002

Figure 3.

Figure 3

Tat neutralizes the inhibitory effect of SIRT1 on NF-κB-responsive promoters

Promoter reporter assays using (A) the IκBα, (B) the E-selectin, and (C) the 3X-κB luciferase reporter construct. Luciferase constructs (0.2 μg) were transiently cotransfected with expression vectors for p65 or p65 K310R (1 ng), SIRT1 or SIRT1 P447E (0.2 μg), and Tat (50 ng) into HeLa cells. The mean of three independent experiments (±SEM) is shown.