Figure 5. ERα and ERβ Gene Expression in GN11 and GT1-7 Cells Treated With 17β-Estradiol.
A) GN11 and B) GT1-7 neuronal cells were treated with 1nM, 10nM or 100nM of E2 for 16 hours. Total RNA was extracted and quantitative real-time PCR was performed with primers specifically designed to amplify mERα and mERβ. GN11 data are expressed as the mean ± SEM (n=6). GT1-7 data are expressed as the mean ± SEM (n=4). *, p < 0.01 vs. control. ** denotes values below detection limit of the assay.