Table I.
Centrosome amplification and pericentriolar satellites in CHO cells exposed for 24 hr to AZT.
| Treatment AZT μM | % cells w/aberrant mitotic figuresab | % cells bearing > 2 centrosomesc | % cells w/supernumerary centrosomes (EM) | %cells w/pericentriolar satellites (EM)d |
|---|---|---|---|---|
| 0 (control) | 0 | 0.2 | 1.1 ± 0.8 | 0.4 ± 0.2 |
| 200 | 0.8 | 0.4 | NAe | NA |
| 400 | 1.8 | 1.2 | 2.8 ± 0.8 | 4.8 ± 0.5 |
| 800 | 5.3 | 1.9 | 4.4 ± 0.5 | 6.9 ± 0.5 |
a
n = 500 mitotic figures, including multipolar mitoses and cells bearing lagging chromosomes
b
mean of 2 experiments
c
n = 200 cells with visible centrosomes
d
n= 50, 60 and 55 cells with visible centrosomes for 0, 200 μM AZT and 400 μM AZT, respectively
e
NA= Not assayed