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. Author manuscript; available in PMC: 2010 May 14.
Published in final edited form as: Vaccine. 2009 Mar 10;27(22):2967–2980. doi: 10.1016/j.vaccine.2009.02.095

Fig. 4. Detection of Tarp in purified EBs but not RBs.

Fig. 4

HeLa cells with (HeLa-D) or without (HeLa) C. trachomatis D organisms, purified EBs or RBs along with the fusion protein GST-Tarp (from serovar D) were resolved in a SDS polyacrylamide gel. The gel-separated protein bands were blotted onto nitrocellulose membrane for Western blot detection of chlamydial proteins including Tarp, CPAF, MOMP and human b-actin with antibodies listed along the left side of the figure. Tarp was detected in both the whole infected cell lysate and purified EB but not RB samples. Tarp protein always displayed degradation fragments regardless of the source as indicated along the right side of the panel a.