Table 1. Summary of human antibody recognition of chlamydial fusion proteins.
antibody reactivity.
| Antigen groupa | Gene name | Function and related information | Frequency of reactivity with antisera: | ||
|---|---|---|---|---|---|
| STIb (24)c | Trachoma (8) | ||||
| Proteins predominantly recognized by human antisera in the current study | New antigens (Not published) | CT456 | Tarp, a chlamydial translocated actin recruiting phosphoprotein, injected into host cell cytosol to facilitate chlamydial invasion of nonphagocytic epithelial cells by targeting host small GTPases and inducing F-actin reorganization | +d (17)e | + (8) |
| CT673 | Pkn5, possible serine/threonine kinase secretable by Salmonella T3SS | + (13) | + (5) | ||
| Antigens also identified in previously published studies | CT089 | CopN, a negative regulator of chlamydial T3SS, immunization with CopN can induce partial protection against C. pneumoniae airway infection [77] | + (23) | + (6) | |
| CT110 | HSP60 [77] | + (17) | + (5) | ||
| CT119 | Inclusion membrane protein A (IncA) [35] | + (19) | − (3) | ||
| CT529 | Inc | + (20) | − (3) | ||
| CT681 | MOMP from serovar D [77] | + (16) | − (1) | ||
| CT813 | Inc | + (21) | − (3) | ||
| CT858 | CPAF, a chlamydial protease involved in immune evasion, able to induce partial protection against C. trachomatis urogenital infection [66, 84] | + (21) | + (6) | ||
| Proteins not recognized by any human antisera | CT606.1 | 79AA, Hypothetical protein | − (0) | − (0) | |
| CT652.1 | 59AA, Hypothetical protein | − (0) | − (0) | ||
| CT671 | 283AA, Hypothetical protein | − (0) | − (0) | ||
| CT672 | FliN flagellar motor switch domain | − (0) | − (0) | ||
| CT718 | Related to FliI & FliF | − (0) | − (0) | ||
| CT847 | 172AA, Hypothetical protein | − (0) | − (0) | ||
| CT848 | 168AA, Hypothetical protein | − (0) | − (0) | ||
| CT860 | Predicted to be CopD2 | − (0) | − (0) | ||
| CT861 | Predicted to be CopB2 | − (0) | − (0) | ||
| CT863 | 482AA, Hypothetical protein | − (0) | − (0) | ||
Ag=antigen, the 55 chlamydial antigens were categorized into 4 different groups based on their reactivity with human antisera and only three groups were listed in the table. The antigens that were positively recognized by one or more human antisera but not immunodominant were not listed.
STI stands for patients with C. trachomatis infection in the urogenital tract while “trachoma” stands for patients with the infection in ocular epithelial tissues.
A total of 24 STI and 8 trachoma antisera were used in the reactivity measurement.
“+” indicates that the antigen was immunodominantly recognized by the human antibody samples while “−” for nondominant or no recognition.
The number of positively reactive antisera for each antigen was listed in the bracket.