Table 3.
Cytokine/Chemokine Production After RSV Challenge of Mice Treated with TLR Agonists during FI-RSV immunization or during RSV challenge
Day After Challenge | Treat at Priming | Treat at Challenge | IL-13c | Eotaxin | IFN-γ | MIP-1α | MIP-1β |
---|---|---|---|---|---|---|---|
Day 4 | PBS | PBS | 420 ± 97 | 1515 ± 533 | 683 ± 143 | 200 ± 62 | 341 ± 67 |
CpG | PBS | 23 ± 7a | 93 ± 35a | 1451 ± 280 | 164 ± 39 | 301 ± 64 | |
TLR7/8 agonist | PBS | 531 ± 39 | 1699 ± 275 | 1155 ± 144 | 306 ± 29 | 488 ± 67 | |
CpG + TLR7/8 | PBS | 110 ± 24a | 397 ± 85 a | 1813 ± 265 | 167 ± 25 | 450 ± 88 | |
PBS | CpG | 1812 ± 42a | 816 ± 73 | 731 ± 145 | 362 ± 36a | 484 ± 30 | |
PBS | TLR7/8 agonist | 288 ± 54 | 848 ± 96 | 1147 ± 412 | 320 ± 53 | 403 ± 41 | |
PBS | CpG + TLR7/8 | 188 ± 48a | 910 ± 141 | 652 ± 106 | 707 ± 108a | 644 ± 105 | |
Day 7 | PBS | PBS | 112 ± 15 | 1587.6 ± 240.4 | 58 ± 8 | 108 ± 9 | 199 ± 38 |
CpG | PBS | ND a,b | 269 ± 18a | 83 ± 15 | 110 ± 10 | 111 ± 12 | |
TLR7/8 agonist | PBS | 220 ± 42 | 2469 ± 432 | 122 ± 20a | 167 ± 25 | 159 ± 25 | |
CpG + TLR7/8 | PBS | 69 ± 39 | 234 ± 42a | 47 ± 4 | 66 ± 6 a | 81 ± 31 | |
PBS | CpG | 107 ± 7 | 2391 ± 271 | 226 ± 31a | 810 ± 27 a | 493 ± 30 a | |
PBS | TLR7/8 agonist | 63 ± 15 | 1588 ± 377 | 137 ± 22a | 192 ± 27 | 158 ± 58 | |
PBS | CpG + TLR7/8 | 103 ± 36 | 1708 ± 209 | 237 ± 21a | 520 ± 45 a | 262 ± 50 |
Statistically significant relative mice treated with PBS at both priming and challenge, p<0.05.
ND = not detected
Changes in IL-4, IL-5, and IL-13 levels paralleled each other. For clarity and conciseness, only IL-13 data are shown as representative of type 2 cytokine production.