Effect of Sac1 digestion and mutation of the trans splice acceptor on activity of the BmRPL6 and BmRPL 13 promoters
Transfections and luciferase reporter assays were conducted as described in Materials and Methods. Activities are expressed as a percentage of the mean activity of three independent transfections normalized to embryos transfected with the BmRPS12 promoter carried out in parallel, as described in Materials and Methods. Bars represent the mean activity and error bars the standard deviation of six independent transfections. Patterns highlight the groups of promoter constructs analyzed, as in previous figures.