C57BL/6 mice were immunized with BCG, or with BCG and indicated lactoferrins, and aerosol challenged with <100 CFU Erdman MTB. Mice were assessed for organ bacterial load after 30 days. Mice immunized with BCG and human sialylated lactoferrin (rhLF-Sial) showed the greatest reductions in lung CFU, with reduced dissemination to other tissue. Comparisons are shown against non-immunized mice, immunization with BCG alone, human milk-derived lactoferrin (hLF), or with the non-sialylated form (rhLF-UnS). Results are shown as average ± standard deviation for 5–10 mice per group. *p<0.05, compared to non-immunized controls.