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Co-knockdown of Zn72D and bel restored MLE protein levels to 70% of normal levels. (A) One representative quantitative western blot for MLE protein upon knockdown of mle, zn72d, bel, or zn72d+bel. (B) MLE levels were quantitated by first normalizing to the amount of γ-tubulin and then averaging the numbers obtained from the 3 experiments with 2 technical replicates per experiment. Standard deviation was found for the average of each experiment.
