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. 2009 Apr 3;10(5):474–479. doi: 10.1038/embor.2009.34

Figure 1.

Figure 1

TatABC subunits support export and maturation of pre-NrfC mutants in ΔtatABCDE cells, but not their turnover. Pre-NrfC and the M1 and M2 variants were expressed from the arabinose-inducible pBAD24 plasmid in wild-type (WT) MC4100 cells or from the ΔtatABCDE cells expressing TatABC from the pEXT22 plasmid as indicated. Cells were analysed at times (in hours) after induction with arabinose, and immunoblotted using antibodies to the 6-His tag on pre-NrfC. Precursor and mature forms are indicated (Pre and Mat), and mobility of a 25-kDa marker is shown. NrfC bands were quantified by densitometry and are shown in the graph, in which data for pre-NrfC, M1 and M2 are indicated after expression in wild-type cells (filled symbols) or in ΔtatABCDE cells expressing TatABC (open symbols).