Figure 2.

FeS mutant forms of NrfC are stable in ΔtatD cells. Pre-NrfC, M1 and M2 were expressed in wild-type (WT) and ΔtatD cells. Cells were analysed at times (in hours) after the induction of synthesis with arabinose as in Fig 1, and the graph shows the amounts of protein present (from quantitative immunoblotting) over time. The data for the expression of M1 and M2 in wild-type cells are denoted by filled triangles and circles, respectively, and data for other samples are indicated on the graph. Immunoblots are shown with precursor and mature forms (Pre and Mat).