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. 2009 Apr 3;10(5):474–479. doi: 10.1038/embor.2009.34

Figure 3.

Figure 3

Mutated pre-NapG lacking an FeS cluster is rapidly degraded in wild-type cells but stable in ΔtatABCDE cells expressing TatABC or in ΔtatD cells. Pre-NapG was expressed from the pBAD24 vector in WT cells, ΔtatABCDE cells that co-express TatABC from the pEXT22 plasmid, and ΔtatD cells as indicated. Samples were analysed at times (in hours) after the addition of arabinose to induce synthesis. A mutated version (preNapG-M1) lacking an FeS ligand was analysed simultaneously. Mobilities of precursor and mature NapG (Pre and Mat, respectively) and a 25-kDa marker are shown. WT, wild type.