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Overexpression of TatD leads to enhanced stability of NrfC and inhibition of export. His-tagged TatD was expressed in wild-type (WT) and ΔtatD cells together with pre-NrfC or the M2 mutant. Samples were immunoblotted for NrfC at times (in hours) after the induction of NrfC synthesis. Mobility of the His-tagged TatD is also indicated. Control panel: the same cells were analysed without the overexpression of TatD. Mat, matured; Pre, precursor.
