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TatD directs the rapid turnover of a non-FeS Tat substrate, pre-FhuD. Pre-FhuD was expressed from the pBAD24 plasmid in Wt, ΔtatD cells and ΔtatABCDE cells expressing TatABC. Samples were also analysed from the same cell types expressing pre-FhuD and TatD from the pEXT22 plasmid.
