Table 3.
Msp1 and msp2 genotyping by CE and gel electrophoresis of 240 microscopy P. falciparum-positive samples from asymptomatic children
Capillary electrophoresis | Gel electrophoresis | |||||||||||||
msp1 | msp2 | msp1 | msp2 | |||||||||||
K1 | MAD20 | RO33 | total | FC27 | IC | total | K1 | MAD20 | RO33 | total | FC27 | IC | total | |
PCR positive | 195 | 129 | 124 | 223 | 169 | 204 | 223 | 197 | 129 | 126 | 223 | 166 | 201 | 223 |
Fragmentsa | 314 | 160 | 124 | 598 | 230 | 415 | 645 | 225 | 147 | 126 | 498 | 191 | 253 | 444 |
Frequencyb | 33 | 14 | 1 | 48 | 20 | 76 | 96 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
Meanc (95%CI) |
1.61 | 1.24 | 1.0 | 2.68 (2.50–2.86) |
1.37 | 2.03 | 2.89 (2.66–3.13) |
1.14 | 1.14 | 1.0 | 2.23 (2.11–2.36) |
1.15 | 1.26 | 1.99 (1.89–2.09) |
a Total number of detected msp1 and msp2 fragments
b number of different alleles detected in all samples, size bins of 3 bp
c Number of alleles/sample, excluding all parasite-negative samples
n.d. not determined