Table 1.
Primer sequences used for Q-RT-PCR and in situ hybridization
| Gene | Symbol | Presumed Process/Function | Contig ID | Strand | Sequence | Method |
|---|---|---|---|---|---|---|
| Epithelial Membrane Protein 1 | EMP1 | Proliferation/differentiation | TC00532 | F | CTTGGCCATCATCTTTGG | PCR |
| Epithelial Membrane Protein 1 | EMP1 | Proliferation/differentiation | TC00532 | R | AAGAAGCGGCATCCTTTC | PCR |
| Glutamate-ammonia Ligase | GLUL | Nitrogen metabolism | TC00765 | F | ACTTTGGCGTTGTGGCTA | PCR |
| Glutamate-ammonia Ligase | GLUL | Nitrogen metabolism | TC00765 | R | TGGCTTCCGTGCTGTAGT | PCR |
| Keratin 14 | KRT14 | Intermediate filament | MC00279 | F | GCAGCTGACCGATTTGAG | PCR |
| Keratin 14 | KRT14 | Intermediate filament | MC00279 | R | CCAGCCGTGACTTGATGT | PCR |
| Keratin 14 | KRT14 | Intermediate filament | MC00279 | F | AATTAACCCTCACTAAAGGGAGATGCAGTCCCAACTGTCAATG | ISH |
| Keratin 14 | KRT14 | Intermediate filament | MC00279 | R | ATTTAGGTGACACTATAGAAGAGATCCAGCCAGGTGTTGGTAG | ISH |
| Keratin 6A | KRT6A | Intermediate filament | MC02467 | F | CCCTGCAGAAAGCCAAG | PCR |
| Keratin 6A | KRT6A | Intermediate filament | MC02467 | R | TGTCTAGGGCCAGCTTGA | PCR |
| Keratin 6A | KRT6A | Intermediate filament | MC02467 | F | AATTAACCCTCACTAAAGGGAGACGTTTGGATGGTGATCTGAG | ISH |
| Keratin 6A | KRT6A | Intermediate filament | MC02467 | R | ATTTAGGTGACACTATAGAAGAGTCCTGGTAGTCACGCAGTTG | ISH |
| Matrix Metalloproteinase 1 | MMP1 | Collagen degradation | MC01311 | F | CACCCGAAATCTGTGACAA | PCR |
| Matrix Metalloproteinase 1 | MMP1 | Collagen degradation | MC01311 | R | CGGCGCCAGAAGAATC | PCR |
| Matrix Metalloproteinase 1 | MMP1 | Collagen degradation | MC01311 | F | AATTAACCCTCACTAAAGGGAGACAGGAGCTCAGGGAAGAATG | ISH |
| Matrix Metalloproteinase 1 | MMP1 | Collagen degradation | MC01311 | R | ATTTAGGTGACACTATAGAAGAGAAGTGGACGTCTCCACCAAC | ISH |
| Thyroid Hormone Receptor α | TR-α | Transcription factor | N/A | F | GTCGGATGCCATCTTTGA | PCR |
| Thyroid Hormone Receptor α | TR-α | Transcription factor | N/A | R | CAGCACCGCCTGAAGTAG | PCR |
| Thyroid Hormone Receptor β | TR-β | Transcription factor | N/A | F | AACGCTGAATGGGGAAAT | PCR |
| Thyroid Hormone Receptor β | TR-β | Transcription factor | N/A | R | CGACATCCCAAGGTCAAA | PCR |
| Uromodulin | UMOD | Protection/barrier | MC01737 | F | GCGGATTCATTGACCATTC | PCR |
| Uromodulin | UMOD | Protection/barrier | MC01737 | R | TCGGAGTTGGTGATCTTGA | PCR |
| Uromodulin | UMOD | Protection/barrier | MC01737 | F | AATTAACCCTCACTAAAGGGAGATCCTGCTCCTACCCTCTCAA | ISH |
| Uromodulin | UMOD | Protection/barrier | MC01737 | R | ATTTAGGTGACACTATAGAAGAGGGTCACGGTGGACATTCTCT | ISH |
| Calmodulin 2 | CALM2 | Ca regulation/signaling | MC10283 | F | CCCGTATCTGCGTTTATCC | PCR |
| Calmodulin 2 | CALM 2 | Ca regulation/signaling | MC10283 | R | CTTAACGGAAGGTGGTTGG | PCR |
| Calmodulin 2 | CALM2 | Ca regulation/signaling | MC10283 | F | AATTAACCCTCACTAAAGGGAGAAAGGACTGGCTTGCAGTAGG | ISH |
| Calmodulin 2 | CALM2 | Ca regulation/signaling | MC10283 | R | ATTTAGGTGACACTATAGAAGAGAGGCATAACGCCAGCTAAGA | ISH |
| Transcriptional Intermediary Factor 1 | TIF1 | Control gene | MC00782 | F | CCTCTGGTCTCGGATCG | PCR |
| Transcriptional Intermediary Factor 1 | TIF1 | Control gene | MC00782 | R | TCCTGCGGAGGCTCTT | PCR |
PCR refers to Q-RT-PCR. Contig ID is the Sal-Site (www.ambystoma.org) identifier associated with each gene. Strand refers to the strand to which primer design was targeted. F = forward strand primer and R = reverse strand primer. Primers for TR-α and TR-β were designed from sequences obtained from the National Center for Biotechnology Information. For the “Contig ID” column, N/A implies that a gene does not have a Sal-Site contig ID.