Table 2.
Sequence of PNA probes
| Name | Sequence |
|---|---|
| PNA-free (WT990) | Gly-D-Ala-Gly-Gly-Aba- (Gly) 4-D (Cys-Ser-Lys-Cys) |
| MYCC | |
| MYCC PNA antisense (WT4219) | Gly-D-Ala-Gly-Gly-Aba-GCATCGTCGCGG-AEEA-D (Cys-Ser-Lys-Cys) |
| MYCC PNA mismatch (WT4235) | Gly-D-Ala-Gly-Gly-Aba-GCATGTCTGCGG-AEEA-D (Cys-Ser-Lys-Cys) |
| CCND1 | |
| CCND1 PNA antisense (WT4185) | Gly-D-Ala-Gly-Gly-Aba-CTGGTGTTCCAT-AEEA-D (Cys-Ser-Lys-Cys) |
| CCND1 PNA mismatch (WT4172) | Gly-D-Ala-Gly-Gly-Aba-CTGGACAACCAT-AEEA-D (Cys-Ser-Lys-Cys) |
| CCND1 peptide mismatch (WT4113) | Gly-D-Ala-Gly-Gly-Aba-CTGGTGTTCCAT-AEEA-D (Cys-Ala-Ala-Cys) |
| Fl-CCND1 PNA antisense (WT4433) | SFX-AEEA-CTGGTGTTCCAT-AEEA-D (Cys-Ser-Lys-Cys) |
| Fl-CCND1 peptide mismatch (WT4361) | SFX-AEEA-CTGGTGTTCCAT-AEEA-D (Cys-Ala-Ala-Cys) |
| KRAS | |
| KRAS PNA antisense | SBTG2-DAP-AEEA-GCCAACAGCTCC-AEEA-D (Cys-Ser-Lys-Cys) |
| KRAS PNA antisense (WT4286, Asp 12 mutant) | DOTA-AEEA-GCCATCAGCTCC-AEEA-D (Cys-Ser-Lys-Cys) |
| KRAS PNA, 1 mismatch (WT4271, Gly12 wild type) | DOTA-AEEA-GCCACAGCTCC-AEEA-D (Cys-Ser-Lys-Cys) |
| KRAS PNA, 1 mismatch (WT4295, Val12 mutant) | DOTA-AEEA-GCCACAGCTCC-AEEA-D (Cys-Ser-Lys-Cys) |
| KRAS PNA, 2 mismatch (WT4292, Lys 12 mutant) | DOTA-AEEA-GCCTAGCTCC-AEEA-D (Cys-Ser-Lys-Cys) |
| KRAS PNA, 3 mismatch (WT4277, Glu12 mutant) | DOTA-AEEA-GCCTTTGCACC-AEEA-D (Cys-Ser-Lys-Cys) |
| KRAS PNA antisense, peptide mismatch (WT4214, Asp12 mutant) | DOTA-AEEA-GCCATCAGCTCC-AEEA-D (Cys-Ala-Ala-Cys) |
| F1= Fluoresceinyl; SFX= 6-(fluorescein-5-carboxamido) hexanoyl | |