Supplementary Fig. S4.

Immunofluorescence analysis of secreted from muscle stage larvae (SML)-1. Frozen sections were cut from Trichinella spiralis infected rat tissue collected 24 days p.i., fixed in methanol and then probed with anti-SML-1. SML-1 protein was visualised with tetramethyl rhodamine iso-thiocyanate (TRITC)-conjugated anti-mouse IgG (red). Nuclei within the tissue were visualised with DAPI (blue). The single channel (black and white) and an overlay image (colour) are shown. Localisation to the nematode (green arrows), nurse cell hypertrophic nuclei (red arrows) and an unidentified structure on the edge of the nurse cell (purple arrows) are highlighted. Pre-bleed controls are shown in Supplementary Fig. S14. Scale bars = 40 μm.