Table 1. Primers used for mutant construction, complementation and RT-PCR analyses.
| Gene | Purpose | Primer Name | Primer Sequence (5′ - 3′) |
| omcZ | mutant construction | 2076-1 | ATGTGATGCGATATCCCGGC |
| 2076-2 | CGCTGACGTGACACTCGAGAC | ||
| 2076-3 | GTCTCGAGTGTCACGTCAGCGAGTGCCACCTGGGATGAATG | ||
| 2076-4 | GGTGATGCGGAGCTCGTAGATGGCAGGTTGGGCGTCGC | ||
| 2076-5 | CTACGAGCTCCGCATCACC | ||
| 2076-6 | CACCCAGAGGAGGCAGCAGG | ||
| GSU1497 | mutant construction | 1497rg1 | CGAACTTTTGAAGCTTACGTTCC |
| 1497rg2R1 | GATAGCGGAATTCCATGGTTTCCTCCAGTATG | ||
| 1497rg3H3 | GTATGCAAGCTTTAGTCATATCGGATAACTGATTG | ||
| 1497rg4C | GAAACCCACACCACAGATTCCGTATTGAGCG | ||
| rgKANR15′ | GCATGAGAATTCCTGACGGAACAGCGGGAAGTCCAGC | ||
| rgKANRevH3 | GCTATGAAGCTTTCATAGAAGGCGGCGGTGGAATCGAA- | ||
| Rg2076R1 | GCATATGAATTCAATTCAAGAAAGGAGCAGAAAGGAATG | ||
| Rg2076H3 | GCATCAAAGCTTTACCGTTTGACTTTCTTCGGAGC | ||
| GSU2075 & omcZ | operon testing | 2076RT-F | CCCAGGCAAACGTCATTTCC |
| 2075RT-R | CAATGGACGAAAGCACGAAG | ||
| pilA | q-RT | RT_ORF02545_F | CCAACACAAGCAGCAAAAAG |
| RT_ORF02545_R | GCAGCGAGAATACCGATGAT | ||
| omcB | q-RT | RT_ORF04536_F | GACACGGTCAACCAGAACAA |
| RT_ORF04536_R | GGTCCCAGTTTACGACAGGA | ||
| omcE | q-RT | RT_ORF01030_F | CTCGTCCAGCAGCATGAATA |
| RT_ORF01030_R | GGGGTGATCATTGCTCAGAT | ||
| omcS | q-RT | RT_ORF04142_F | CAACCTGGCATACGAGTTCA |
| RT_ORF04142_R | CCATAGTAGGCAGCGGTCAT | ||
| omcZ | q-RT | RT_ORF03438_F | CACGAGCCTGACACTCACTC |
| RT_ORF03438_R | AAGGTTGCTGACCTTGTTGG | ||
| GSU1497 | q-RT | RT_ORF02546_F | ATGGGTGGCAAGGACTTTA |
| RT_ORF02546_R | AACACCCGGTTACCAGAAGA |